Cloning and biochemical characterization of the hectochlorin biosynthetic gene cluster from the marine cyanobacterium Lyngbya majuscula 公开 Deposited Apr 28, · ️️Biochemical Biosynthetic Characterization Cloning Cluster Cyanobacterium Dissertation From Gene Hec» Customized essay writing ️️ Japan⭐ / Custom wrting⭐ Dissertation Writing Services in UAE Dubai, AbuDhabi, Sharjah, UK, USA, Australia, Singapore, India, Qatar, KSA, HongKong. Research websites⚡:: Essay writers for hire. ️️47%(K) Cloning and Biochemical Characterization of the Hectochiorin Biosynthetic Gene Cluster from the Marine Cyanobacterium Lyngbya majuscula by Aishwarya V. Ramaswamy A DISSERTATION submitted to Oregon State University in partial fulfillment of the requirements for the degree of Doctor of Philosophy Presented June 02, Commencement June
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可下载的内容 下载PDF文件. 引文: EndNote MLA APA Chicago. Copy link. Abstract Cyanobacteria are rich in biologically active secondary metabolites, many of which have potential application as anticancer or antimicrobial drugs or as useful probes in cell biology studies.
A Jamaican isolate of the marine cyanobacterium, Lyngbya majuscula was the source of a novel antifungal and cytotoxic secondary metabolite, hectochlorin. Unique features of hectochlorin such as the presence of a gem dichloro functionality and two 2,3-dihydroxy isovaleric acid prompted efforts to clone and characterize the gene cluster involved in hectochlorin biosynthesis.
This gene cluster was designated as a cryptic gene cluster because a corresponding metabolite remains as yet unidentified. The expression of this gene cluster was successfully demonstrated using RT-PCR and these results form the basis for characterizing the metabolite using a novel interdisciplinary approach.
A 38 kb region putatively involved in the biosynthesis of hectochlorin has also been isolated and characterized. The hct gene cluster consists of eight open reading frames ORFs and appears to be colinear with regard to hectochlorin biosynthesis. An unusual feature of this gene cluster includes the presence of a ketoreductase domain in an NRPS module and appears to be the first report of such an occurrence in a cyanobacterial secondary metabolite gene cluster. Other tailoring enzymes present in the gene cluster are two cytochrome P monooxygenases and a putative halogenase.
The juxtaposition of two ORF's with identical modular organization suggests that this gene cluster may have resulted from a gene duplication event. Furthermore, biochemical characterization of two adenylation domains from this cluster strengthens its involvement in the biosynthesis of hectochlorin.
Degree Field Microbiology Degree Grantor Oregon State University Commencement Year Advisor Gerwick, William H. Committee Member Zabriskie, Mark Stone, biochemical biosynthetic characterization cloning cluster cyanobacterium dissertation from gene hec, Jeff Constantine, George Giovannoni, Steve Academic Affiliation Microbiology Non-Academic Affiliation Oregon State University.
Graduate School Subject Marine metabolites -- Synthesis Lyngbya 权利声明 Copyright Not Evaluated Publisher Oregon State University Peer Reviewed No Language English [eng] Digitization Specifications File scanned at ppi Monochrome, Grayscale, bit Color using Capture Perfect 3. CVista PdfCompressor 4. provenance : Submitted by Kirsten Clark clarkkir onid. edu on TZ No. of bitstreams: 1 RamaswamyAishwaryaV pdf: bytes, checksum: 25ede61cfcfcbd MD5 description.
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Ramaswamy, Aishwarya V. Cyanobacteria are rich in biologically active secondary metabolites, many of which have potential application as anticancer biochemical biosynthetic characterization cloning cluster cyanobacterium dissertation from gene hec antimicrobial drugs or as useful probes in cell biology studies.
Doctor of Philosophy Ph. Oregon State University. Gerwick, William H. Zabriskie, Mark Stone, Jeff Constantine, George Giovannoni, biochemical biosynthetic characterization cloning cluster cyanobacterium dissertation from gene hec, Steve.
Graduate School. Marine metabolites -- Synthesis Lyngbya. Copyright Not Evaluated. English [eng]. File scanned at ppi Monochrome, Grayscale, bit Color using Capture Perfect 3. Press to 选择一个动作 下载.
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Graduate Thesis Or Dissertation Cloning and biochemical characterization of the hectochlorin biosynthetic gene cluster from the marine cyanobacterium Lyngbya majuscula Público Deposited. Analytics. Conteúdo disponível para baixar Baixar PDF Graduate Thesis Or Dissertation Cloning and biochemical characterization of the hectochlorin biosynthetic gene cluster from the marine cyanobacterium Lyngbya majuscula Public Deposited. Analytics. Downloadable Content Download PDF. Cited by: Cloning and Biochemical Characterization of the Hectochiorin Biosynthetic Gene Cluster from the Marine Cyanobacterium Lyngbya majuscula by Aishwarya V. Ramaswamy A DISSERTATION submitted to Oregon State University in partial fulfillment of the requirements for the degree of Doctor of Philosophy Presented June 02, Commencement June
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